Submitted to: Bemisia International Workshop Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 10, 2003
Publication Date: March 13, 2003
Citation: McKenzie, C.L., Sinisterra, X., Hunter, W.B., Shatters, R.G. 2003. Begomovirus replication in Bemisia tabaci. In: Proceedings of the Bemisia International Workshop, December 3-8, Duck Key, Florida. Technical Abstract: Geminiviruses affect a large number of crops worldwide and can cause dramatic yield losses. Begomoviruses are one of the three genera described in the Geminiviridae family and are transmitted by the whitefly, Bemisia tabaci, in a persistent manner. Herein we report on the virus-vector interactions of two begomoviruses, the bipartite tomato mottle virus (ToMoV) and the monopartite tomato yellow leaf curl virus (TYLCV). Studies were performed on whiteflies maintained on virus infected tomato plants and transferred to cotton (a virus nonhost). Transcripts of three virus genes representing genomic and complementary strand products were detected and quantified via real-time RT PCR in the whitefly vector after 4 and 7 days of continuous feeding on cotton. Results indicate virus transcriptional differences in the interactions between ToMoV and TYLCV within their vector.