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United States Department of Agriculture

Agricultural Research Service

Title: Rapid and Efficient Production of the Pyrenophora Tritici-Repentis Teleomorph.

Authors
item Friesen, Timothy
item Ali, S. - PLNT PATH NDSU, FARGO, ND
item Stack, R. - PLNT PATH NDSU, FARGO, ND
item Francl, L. - PLNT PATH NDSU, FARGO, ND
item Rasmussen, J. - PLNT PATH NDSU, FARGO, ND

Submitted to: Canadian Journal of Botany
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 2, 2003
Publication Date: December 20, 2003
Citation: FRIESEN, T.L., ALI, S., STACK, R.W., FRANCL, L.J., RASMUSSEN, J.B. RAPID AND EFFICIENT PRODUCTION OF THE PYRENOPHORA TRITICI-REPENTIS TELEOMORPH.. CANADIAN JOURNAL OF BOTANY. 2003. VOL. 81:890-895.

Interpretive Summary: Pyrenophora tritici-repentis is the fungus that causes tan spot of wheat. Tan spot is an economically important foliar disease that causes yield loss to wheat crops throughout the world. In order to better study the genetics of disease caused by the fungus we have identified parameters required for fast, efficient production of the sexual stage of this fungus in culture. Parameters tested include light cycle, temperature and growth medium. Optimum parameters include incubating the fungus on maize leaves placed on water agar in continuous darkness for 12 days followed by a 12 h photoperiod all at 100% relative humidity. This resulted in sexual stage maturity (ascospore discharge) in 29 days. This technique will be helpful to researchers studying the biology of sexual stage development, disease epidemiology and genetics of this fungus.

Technical Abstract:

Last Modified: 9/22/2014
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