|Hogenhout, S - OHIO STATE UNIVERSITY|
Submitted to: Virus Diseases of the Poaceae
Publication Type: Book / Chapter
Publication Acceptance Date: January 28, 2006
Publication Date: N/A
Technical Abstract: The report outlines the salient features of maize fine streak virus (MFSV) including a general description of the causal virus species, virion properties, genome description, the relationship of the virus to other taxa, biological properties of the disease and agronomic aspects of the disease. Maize fine streak virus was isolated from sweetcorn grown in Georgia, U.S.A. It has typical rhabdovirus morphology. Three proteins of 82, 50 and 32 kDa and a single-stranded RNA of ca. 13 kb were detected in purified virions. Sequence analysis indicated the viral genome consists of a negative sense, single stranded-RNA similar to other rhabdoviruses. Current information indicates the presence of 7 open reading frames in the genome. MFSV is serologically distinct from Maize mosaic virus, Maize Iranian mosaic virus, and Wheat American striate mosaic virus. MFSV is serologically related to Sorghum stunt mosaic virus, but has a different vector and host range. MFSV belongs to the genus Nucleorhabdovirus. Zea mays L. is the only currently identified natural host of MFSV. Laboratory hosts of MFSV include barley, oats, rye, bread wheat, barnyard grass (Echiochlnoa crusgalli), annual ryegrass (Lolium multiflorum), giant foxtail (Setaria fabeeri) and green foxtail (Setaria viridis). Insusceptible hosts include brome grass (Bromus inermis), orchardgrass (Daclitys glomerata), quackgrass (Elytrigia repens), timothy (Phleum pratense), Kentucky bluegrass (Poa pratensis), Johnson grass (Sorghum halepense), and sorghum (Sorghum vulgare). MFSV is not transmitted by leaf rub inoculation, but is readily transmitted mechanically by kernel vascular puncture inoculation. The virus is transmitted by Graminella nigrifrons in a persistent manner. The virus can be detected by leaf-dip preparations or by serological techniques (DAS-ELISA, Western blot analysis). Symptoms in the field include fine chlorotic streaks along the major leaf veins. Some maize germplasm, particularly sweet corn hybrids, showed a high incidence of severe symptoms, while other germplasm, primarily of tropical origin, showed a low incidence of much milder symptoms. Maize lines resistant to Maize mosaic virus were also resistant to MFSV.