Submitted to: Ecology Symposium
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2002
Publication Date: N/A
Technical Abstract:
Thlaspi caerulescens, formerly known as T. alpestre, has been reported to be self-pollinated. Among T. alpestre plants, Riley (1956) found only 5.25 % outcrossing, whereas Holmgren (1971) found a range of 5-35% outcrossing. These studies suggest that T. caerulescens plants are predominantly self-pollinated. However, results regarding the mode of pollination for both Thlaspi species have not been confirmed using DNA analysis. Determining the level of homozygosity of plant material is necessary for experiments involving mating designs, genetic diversity, gene linkage and gene expression. In order to determine the mode of pollination of T. caerulescens, two populations have been examined using PCR RAPD markers. Plants of super-Cd accumulators from St. Felix and Viviez, France were scored. DNA polymorphisms among the siblings from the same mother plant indicate that these T. caerulescens populations have a higher level of outcrossing than previously reported of T.alpestre and are not inbred populations. Results from the RAPD analysis of these two populations will be presented. Literature Cited: Holmgren, P. K. (1971). "A biosystematic study of North American Thlaspi montanum and its allies." Mem. New York Botan. Gard. 21:1-106. Riley, R. (1956). "The influence of the breeding system on the genecology of Thlaspi alpestre L." New Phytol 55:319-330.
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