Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 3, 2003
Publication Date: September 16, 2003
Citation: Hammond, J., Hammond, R. 2003. The complete nucleotide sequence of bean yellow mosaic virus isolate bymv-gdd, and comparison to other potyviruses. Archives of Virology. 148:2461-2470. Interpretive Summary: Examination of the differences between plant virus isolates that differ in host range or symptom severity can lead to an understanding of the disease process, and potentially to identification of ways to protect the crop from virus infection. Bean yellow mosaic potyvirus (BYMV) infects a variety of ornamental and field crops, including gladiolus, bulbous iris, gentians, orchids, clover, beans, and peas. Isolate GDD of BYMV (BYMV-GDD) from gladiolus was fully cloned and sequenced for comparison to two distinct isolates from broad bean that differ in host range and symptom expression. Isolate BYMV-MB4 has typical symptoms and host range, while isolate BYMV-S differs in host range and has atypical symptoms. The genomic sequence of BYMV-GDD was found to be more similar to BYMV-MB4 than to BYMV-S; differences between the sequences were concentrated in particular regions (genes) of the genome. Each of these variable genes have previously been correlated with symptom or host-range differences in other potyviruses, and may contribute to the atypical nature of BYMV-S. Creation of an infectious clone of BYMV-GDD will allow exchange of regions between different BYMV isolates to determine which of the viral genes affect host range or symptoms, etc., as the basis for improving crop resistance.
Technical Abstract: The complete nucleotide sequence of Bean yellow mosaic virus (BYMV) gladiolus isolate GDD was determined and compared to broad bean isolates BYMV-MB4 and BYMV-S. The BYMV-GDD genome (9528nt) was more similar to BYMV-MB4 (9532nt) than to BYMV-S (9547nt), which has atypical symptom expression and host range. The greatest variability occurred in the 5 untranslated region, P1 protein, and NIa-VPg protein, the N-terminal two thirds of HC-Pro, and the C-terminal one third of P3. Each of these regions has been correlated with symptom or host differences between isolates of other potyviruses, and may contribute to the atypical nature of BYMV-S.