ARS | Publication request: REGISTRATION OF ANTHRACNOSE RESISTANT PINTO BEAN GERMPLASM LINE USPT-ANT-1

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 28, 2003
Publication Date: August 1, 2003
Citation: Miklas, P.N., Kelly, J.D., Singh, S. 2003. Registration of anthracnose resistant pinto bean germplasm line uspt-ant-1. Crop Science 43:1889-1890.

Interpretive Summary: Pinto bean germplasm line USPT-ANT-1 with anthracnose resistance was rapidly developed by marker-assisted selection to combat the emerging anthracnose disease problem in North Dakota and Minnesota. USPT-ANT-1, released in 2002, derived from a collaborative effort lead by USDA-ARS (Prosser, WA), in cooperation with the Agricultural Experiment Stations of Idaho, Michigan, North Dakota, and Washington. The benefit of this line is that it possesses the Co-42 gene which confers resistance to all known North American strains of Colletrotrichum lindemuthianum that causes anthracnose, a major seed-borne disease of beans, that is endemic to Michigan, New York and Ontario. Anthracnose was observed in pinto bean fields in North Dakota and Minnesota in 2001, placing 350,000 acres of susceptible pinto bean production at risk. Marker-assisted backcrossing using a DNA marker tightly linked with Co-42 was used to rapidly introgress the gene into the highly susceptible pinto bean background. The USPT-ANT-1 germplasm will be critical to combating future outbreaks of anthracnose disease in the Upper Midwest.

Technical Abstract: USPT-ANT-1 derives from a ¿modified¿ backcrossing scheme (Pinto *4/SEL 1308); modified, because a different pinto parent was used for each backcross. Thus, USPT-ANT-1 is a modified BC4F2:5 bulk from the cross USPT-CBB-1/4/`Buster¿/OT9743-586-1 (F1)/3/`Maverick¿// `Othello¿ /SEL 1308. The tropical black-seeded line SEL 1308 from Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia is the donor of Co-42. USPT-CBB-1, OT9743-586-1, Buster, Maverick, and Othello are pinto bean breeding lines and cultivars. The crossing and marker-assisted selection work was conducted by USDA-ARS in Prosser (WA) in the absence of disease pressure. Resistance of USPT-ANT-1 in the presence of the disease was confirmed in pathogen screening tests conducted in the greenhouse at Michigan State University. USPT-ANT-1 also possesses resistance to bean rust and bean common mosaic virus, which are major bean diseases in the Midwest and seed production region of the Northwest, respectively. This line will be most useful for incorporating resistance to anthracnose primarily in the pinto bean market class, but also in the medium-seeded great northern, pink, and small red market classes as well.