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United States Department of Agriculture

Agricultural Research Service

Title: Decay of Estrogenic Activity in Municipal Wastewater Lagoons.

Author
item SHAPPELL, NANCY

Submitted to: SETAC Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 2003
Publication Date: September 28, 2003
Citation: Shappell, N.W. 2003. Decay of estrogenic activity in municipal wastewater lagoons. Society of Environmental Toxicology and Chemistry Asia/Pacific & Australasian Society for Ecotoxicology Meeting, Sept. 28-Oct. 2, 2003, Christchurch, New Zealand.

Technical Abstract: Estrogenic activity of wastewater held in municipal lagoons was monitored over an ~ three month period, using the MCF-7 cell line in a modified E-screen. One lagoon was emptied and refilled with fresh wastewater effluent over a one month period to reach levels equivalent to a second lagoon that had not received wastewater effluent since the previous year. The effluent fill estradiol equivalent concentration ranged from 76 (initial) to 35 X 10-13M (final). Water was sampled on day 0, 4, 8, 16, 28, 42, and 84 post fill and extracted (250ml)using an Oasis column, eluted with methanol, ethyl acetate, methylene chloride, and tert-butyl ether. Estradiol equivalents in lagoon wastewater fell from 24.2 X 10-13M to 5.6 X 10-13M after 84 days, with a T1/2 of ~ eight days. A similar half life of estradiol equivalents was found in the lagoon that had not received fresh effluent, falling from 3.42 X 10-13M to 1.68 X 10-13M in eight days. As ambient temperature rose, algal growth increased and estrogenic activity increased. Samples that were extracted subsequent to freezing were found to give dissimilar results. Possible explanations include lysis and extraction of bacterial and or algal components.

Last Modified: 9/10/2014
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