ARS | Publication request: ELUCIDATION OF THE INCOMPATIBLE AND COMPATIBLE BARLEY-POWDERY MILDEW INTERACTIONS USING AFFYMETRIX GENECHIPS: A MICROARRAY REPRESENTATION OF THE QUADRATIC CHECK

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2003
Publication Date: January 15, 2003
Citation: Caldo, R.A., Wise, R.P. 2003. Elucidation of the incompatible and compatible barley-powdery mildew interactions using affymetrix genechips: a microarray representation of the quadratic check. Plant and Animal Genome Conference Proceedings. XI Conference. p. 758.

Technical Abstract: The interaction between plants and their pathogens involves transcriptional modulation of many genes that result in incompatibility (resistance) or compatibility (susceptibility). These differential host responses are usually described using a simplified quadratic check in a gene-for-gene manner. In this study, oligo-based Affymetrix barley microarrays representing 22,500 genes are being used to extend the quadratic check and monitor gene-for-gene interactions on a global scale. Pairwise expression profiling is being performed with plants containing the Mla6 and Mla13 resistance specificities in response to the powdery mildew isolates 5874 (AvrMla6, virMla13) and K1 (AvrMla13, virMla6). Our objective is to identify genes induced/repressed during early stages of pathogen infection to fully elucidate the molecular mechanisms orchestrating incompatible and compatible barley-powdery mildew interactions. Analysis of these expression profiles will provide insights into the early signaling events that occur during infection, facilitating strategies for germplasm improvement.