Submitted to: Weed Science Society of America Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 13, 2002
Publication Date: February 8, 2002
Citation: Nadella, D., Hoffer, B.L., Foley, M.E. 2003. Identification of quantitative trait loci (QTLs) for dormancy in wild oat (Avena fatua). Weed Science Society of America Meeting. February 8-14, 2003. Jacksonville, FL. 43:27.
Wild oat is a wide spread weed causing substantial yield losses in small grain crops. Variable germination of seeds due to dormancy makes the control of wild oat difficult. Dormancy is a complex polygenic character with large influences of the environment on its expression. We are investigating genetic and environmental factors regulating dormancy in wild oat. To this end, a genetic linkage map is being constructed on 126 F2:F9 recombinant inbred (RI) lines derived from the cross M73 (D) x SH430 (ND) to detect dormancy quantitative trait loci (QTLs). So far, 255 amplified fragment length polymorphism (AFLP) loci have been mapped and 28 linkage groups have been identified covering a map length of 1560 cM. Including the 16 unlinked loci, the genome coverage is comparable to that of the cultivated hexaploid oat (<I>Avena sativa</I>). The linkage groups varied in their length from 1.6 cM with 2 loci to 220 cM with 52 loci. A significant effect of temperature on germinability and a genotype by germination temperature interaction have been detected over a range of 10 to 30 C in the RI population. Using bulked segregant analysis, 45 loci showing differential fragment intensities between the dormant and non-dormant bulks have been identified at 15 C. Except for a few, most of the QTLs are sensitive to the germination temperature and are differentially expressed. The interaction of all of these dormancy QTLs with germination temperature is being investigated. firstname.lastname@example.org