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United States Department of Agriculture

Agricultural Research Service

Title: Interferon-Gamma (Ifn) and Tumor Necrosis Factor-Alpha (Tnf) Secretion by Blood Mononuclear Leukocytes (Mnl) from Young and Adult Cattle Vaccinated with Attenuated Mycobacterium Bovis (Bcg): Modulation by 1,25-(Oh)2vitamin D3

item Nonnecke, Brian
item Waters, W
item Horst, Ronald
item Fowler, M - LAND O'LAKES, IA
item Miller, B - LAND O'LAKES, IA

Submitted to: American Dairy Science Association Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 23, 2002
Publication Date: N/A

Technical Abstract: Interferon and TNF are critical in the development of an effective immune response. Vitamin D, essential in short-term calcium homeostasis and recently shown to modulate proliferation and function of lymphocytes from adult cattle, may be an effective modulator of the calf¿s immune system. The present study examined effects of maturity (i.e., calf vs. adult), antigen sensitization, and VitD on IFN and TNF secretion by bovine MNL. Heifers (VA, n=4) and 1-wk-old calves (VC, n=6) were vaccinated with BCG and boosted 6 wk later. Ten weeks after primary vaccination, MNL from vaccinates and nonvaccinated, age-matched calves (NVC, n=4) were evaluated in vitro for their capacity to produce IFN and TNF. Cells were nonstimulated (NS) or stimulated with mitogen (PWM) and antigen (PPD) in the presence of VitD (0, 1, and 10 nM). Cytokines in culture supernatants harvested at 20, 44, and 68 h were quantified by ELISA. Calf MNL produced (P less than .10) more IFN and TNF than adult MNL in NS cultures; however, adult MNL produced more IFN (P less than .05) than calf MNL in PWM stimulated cultures. Secretion of TNF in PWM stimulated cultures were unaffected (P=.36).

Last Modified: 4/22/2015
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