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United States Department of Agriculture

Agricultural Research Service

Title: Utilization of Various Carbohydrates by Intestinal Bacteria

Authors
item Holt, Scott - WESTERN IL UNIV
item Miller-Fosmore, C - WESTERN IL UNIV
item Cote, Gregory

Submitted to: American Society for Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 23, 2002
Publication Date: May 23, 2003
Citation: HOLT, S.M., MILLER-FOSMORE, C.M., COTE, G.L. UTILIZATION OF VARIOUS CARBOHYDRATES BY INTESTINAL BACTERIA. AMERICAN SOCIETY FOR MICROBIOLOGY ANNUAL MEETING. 2002. PAPER NO. I-67.

Technical Abstract: Certain carbohydrates have been used as prebiotic dietary supplements to selectively stimulate the growth of beneficial colonic bacteria such as bifidobacteria and lactobacilli. In this study, 6 carbohydrate preparations were screened for selective growth among 13 colonic bacterial species. The carbohydrates used include three new preparations synthesized from alternansucrase (asr; maltose acceptor product, raffinose acceptor product, and low mass alternan), and three were commercial products (Benefiber, Fibersol-2, and Neosugar). Anaerobic growth was determined by absorbance at 600nm. Three Lactobacillus species tested displayed no growth on most of the carbohydrates. L. casei showed growth on maltose acceptor, and L. acidophilus displayed growth on Neosugar. Growth of five bifidobacterial species on the carbohydrates was variable and depended on the species tested. B. adolescentis and B. pseudocatenulatum displayed growth on the maltose and raffinose acceptors. B. pseudocatenulatum also displayed growth on low mass alternan. All the bifidobacterial species tested displayed growth on Neosugar. Bacteroides thetaiotaomicron displayed growth on low mass alternan, raffinose acceptor, and Neosugar. Clostridium perfringens displayed various levels of growth on all the carbohydrates tested except for raffinose acceptor. Enterobacter aerogenes displayed growth on Neosugar. Escherichia coli and Salmonella typhimurium displayed no growth on any of the carbohydrates tested. Growth curves for B. pseudocatenulatum showed the highest absorbance on Neosugar (1.5), followed by glucose (1.4), raffinose acceptor (1.3), and maltose acceptor (0.5). Enzyme profiles using API ZYM tests were determined for B. adolescentis and B. pseudocatenulatum following growth on some of the carbohydrates. In general, glycosidase profiles were similar despite the carbohydrate used for growth including glucose. In summary, five bifidobacterial species displayed various levels of growth on asr-derived oligosaccharides. Enterobacter, Escherichia, and Salmonella species did not show growth on the asr-derived oligosaccharides.

Last Modified: 4/18/2014
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