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United States Department of Agriculture

Agricultural Research Service

Title: Synthetic Approaches in Developing Dna-Based Markers for Crop Improvement Via Marker-Assisted Breeding

Authors
item HU, JINGUO
item Miller, Jerry
item GULYA, THOMAS
item Vick, Brady

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2002
Publication Date: January 1, 2003
Citation: HU, J., MILLER, J.F., GULYA JR, T.J., VICK, B.A. SYNTHETIC APPROACHES IN DEVELOPING DNA-BASED MARKERS FOR CROP IMPROVEMENT VIA MARKER-ASSISTED BREEDING. PLANT AND ANIMAL GENOME ABSTRACTS. 2003. P. 55. ABSTRACT NO. W252.

Interpretive Summary: An extensive volume of information regarding the genome structure of crop plants has been generated during the past decade or more, from the first linkage map of molecular genetic markers in 1986 to the whole genome draft sequence of rice in 2002. A great potential exists for using these data to elucidate the genetic bases of biologically and agriculturally important processes in crop plants. The applied genomics project in our unit focuses on the development and application of strategies that utilize genomic infomation in order to identify user-friendly DNA markers associated with the agronomic traits to be used in germplasm enhancement and crop improvement via marker-assisted selection (MAS). We are using synthetic approaches in DNA marker development: these include the use of pooled by phenotype procedure (PPP), tailed-primer radndom amplified polymorphism (TRAP). These approaches are being used in the development of markers for disease resistance and oil quality of sunflowers and the results will be presented.

Technical Abstract: An extensive volume of information regarding the genome structure of crop plants has been generated during the past decade or more, from the first linkage map of molecular genetic markers in 1986 to the whole genome draft sequence of rice in 2002. A great potential exists for using these data to elucidate the genetic bases of biologically and agriculturally important processes in crop plants. The applied genomics project in our unit focuses on the development and application of strategies that utilize genomic infomation in order to identify user-friendly DNA markers associated with the agronomic traits to be used in germplasm enhancement and crop improvement via marker-assisted selection (MAS). We are using synthetic approaches in DNA marker development: these include the use of pooled by phenotype procedure (PPP), tailed-primer radndom amplified polymorphism (TRAP). These approaches are being used in the development of markers for disease resistance and oil quality of sunflowers and the results will be presented.

Last Modified: 9/10/2014
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