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United States Department of Agriculture

Agricultural Research Service

Title: Molecular characterization of rice wild relatives (Oryza spp.) and accelerating the development of disease resistant germplasm using microsatellite markers

Authors
item Eizenga, Georgia
item Jia, Yulin

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 4, 2002
Publication Date: January 11, 2003
Citation: Eizenga, G.C., Jia, Y. 2003. Molecular characterization of rice wild relatives (Oryza spp.) and accelerating the development of disease resistant germplasm using microsatellite markers. Plant and Animal Genome Conference Proceedings. Jan. 11-15, 2003. San Diego, CA.

Technical Abstract: Wild relatives of crop species are a source of unique genes for crop improvement. Sheath blight (Rhizoctonia solani Kuhn) and blast [Magnaporthe grisea (T.T. Herbert) Yaegashi & Udagawa] are the major diseases of cultivated rice (Oryza sativa L.) in the U.S. A set of 150 markers is being used to characterize a core sample of 576 rice (O. sativa) accessions. The objective of this research is to use these same microsatellite markers to characterize the diversity in approximately 40 A-genome Oryza spp. accessions including O. barthii, O. glumaepatula, O. meridionalis , O. nivara and O. rufipogon. In addition, microsatellite markers which are closely associated with (or tightly linked with) a particular Oryza sp. accession will be used to follow the introgression of the Oryza sp. DNA into cultivated rice. Microsatellite markers which co-segregate with blast and/or sheath blight resistance will be used to follow the introgresssion of novel resistance genes into improved germplasm. Also, the presence of the blast resistance gene, Pi-ta is being monitored using a dominant Pi-ta gene marker.

Last Modified: 9/21/2014
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