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United States Department of Agriculture

Agricultural Research Service

Title: Rapd Markers for the Cr Gene for Resistance to Cronartium Ribicola in Ribes Nigrum

Authors
item Picton, Deric - OREGON STATE UNIV
item HUMMER, KIM
item POSTMAN, JOSEPH

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2002
Publication Date: N/A

Interpretive Summary: White pine blister rust, is a disease from Asia. This disease was introduced into North America about 1890 on infected five-needled pines. Legislation in some eastern states restricts the plalnting of currants, which host the disease. Black currants with a specific gene are imune to this diseaseOur objective was to find genetic markers for this immunity gene. We examined 180 seedlings from a cross of 'Ben Lomond' x 'Consort' (susceptible x hererozygous resistant) seedlings. We used the genetic markers to make a gross map of the Black currant genome. The markers that we found were linked to the Cr gene with an average genetic distance of 30 cM. The low genetic variability determined with RAPD's did not provide a highly resolved map. However, this initial map could be expanded with additional molecular and morphological markers and used for marker assisted selection or to screen cultivated or wild currants for rust-immunity.

Technical Abstract: White pine blister rust, caused by Cronartium ribicola Fisch, is an exotic disease from Asia. This disease was introduced into North America about 1890 on infected five-needled pines (Pinus subsect. Strobus). Legislation in some eastern states restricts Ribes L., the currants, which do-host the disease. The dominant Cr gene from R. ussuriense Jancz. Confers immunity and is used to breed rust-immune black currants. Our objective was to find RAPD markers for this gene. We examined 180 'Ben Lomond' x 'Consort. (susceptible x heterozygous resistant) seedlings. 50 RAPD primers generated an average of 4.7 products with 1.5 polymorphic products (31.9%) of polymorphism). The markers are linked to the Cr gene with an average genetic distance of 30 cM. The low genetic variability determined with RAPD's did not provide a highly resolved map at this time. However, this initial map could be developed with additional molecular and morphological markers and used for marker assisted selection or to screen cultivated or wild currants for rust-immunity.

Last Modified: 7/28/2014
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