ARS | Publication request: ISOLATION AND CHARACTERIZATION OF DIAPAUSE ASSOCIATED TRANSCRIPTS-1, 2 AND 3 FROM THE POTATO PEST, LEPTINOTARSA DECEMLINEATA

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: September 3, 2002
Publication Date: December 3, 2003
Citation: Yocum, G.D. 2003. Isolation and characterization of diapause associated transcripts-1, 2 and 3 from the potato pest, Leptinotarsa decemlineata. Genbank. Accession nos. AF532862-AF532864.

Technical Abstract: Using suppressive subtractive hybridization, fragments of three diapause-associated transcripts (DAT-1, 2 and 3) were isolated from the Colorado potato beetle, Leptinotarsa decemlineata. Full length clones were developed for the transcripts. The nucleotide sequences for LdDAT-1, 2 and 3 were deposited in GenBank and assigned the accession number AF532862, AF532863 and AF532864, respectively. DAT 1 encodes a deduced protein 286 amino acids in length with limited identity to several proteins with leucine-rich domains. DAT-2 encodes a deduced protein 229 amino acids in length with identity to the desiccation stress protein from Tenebrio molitor. DAT-3 encodes a deduced protein 97 amino acids in length with identity to no known protein. DAT-1 and 2 have similar expression patterns as determined by northern blot analysis. Trace levels of these two transcripts are first detected in three day old diapause programmed adults with a significant increase in expression on day 6. Expression of DAT-3 begins on day 12 in diapause programmed adults and expression levels increase until the beetles enter diapause. Expression of DAT-1, 2 and 3 continue at least 60 days into diapause. Minor levels of DAT 1 are observed in day 9 nondiapausing adults, but no expression of DAT-2 or 3 is observed in nondiapausing adults.