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United States Department of Agriculture

Agricultural Research Service

Title: Conversion of Afl Markers to Pcr-Based Sts Markers for the Identification of Heterodera Glycines Populations

item Atibalentja, Ndeme - UNIV OF ILLINOIS
item Noel, Gregory
item Domier, Leslie

Submitted to: Nematology - Abstracts Only
Publication Type: Abstract Only
Publication Acceptance Date: June 7, 2002
Publication Date: N/A

Technical Abstract: Nine AFLP markers were identified that provide varying degrees of specificity to some of the forty eight selected populations involved in a previous study that investigated the parasitic ability of the soybean cyst nematode, H. glycines. The forty eight populations were obtained by subjecting field populations of races 1 through 6, 9, and 14 to several cycles of selection on each of six sources of resistance to H. glycines: Peking, Cloud, PI88788, PI89772, PI209332, and PI90763. The nine AFLP markers were cloned and their DNA nucleotide sequence determined before they were used to design oligonucleotide primers for PCR-amplification of genomic DNA from the original unselected field populations. Following this procedure, we have generated STS (sequence tag site) primers that are specific for races 1 through 6, 9, and 14 of H. glycines. These STS markers should be very useful as diagnostic probes for the identification of field populations of H. glycines.

Last Modified: 4/18/2015
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