Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 10, 2002
Publication Date: November 2, 2002
Citation: Neill, J.D., Ridpath, J.F. Use of serial analysis of gene expression to measure changes in gene expression in MDBK cells following infection with BVDV2. Conference of Research Workers in Animal Diseases. 2002. Abstract No. 184. Technical Abstract: Bovine viral diarrhea virus type 2 (BVDV2) strain 1373 is a virulent strain of BVDV2 that causes high fever, thrombocytopenia, and lymphopenia with accompanying lymphoid depletion and immune suppression. The exact mechanism(s) by which these lesions are brought about is unknown. To investigate the interaction of BVDV2-1373 with the host cell, we used serial analysis of gene expression (SAGE) to identify and quantitate mRNA transcripts in noninfected and BVDV2-1373-infected MDBK cells. SAGE, a sequenced based, global functional genomics technology, allows quantitation of virtually every cellular transcript without prior sequence information. SAGE revealed changes in the expression level of a number of genes in BVDV2-infected cells and that these changes could be placed into categories based on biochemical function. With the exception of a 7-fold decrease in transcription of cytochrome c, little change in expression was seen in genes involved in energy metabolism. Transcripts encoding cellular signaling proteins showed a consistent decline in transcription, indicating possible impaired function of these pathways. This was particularly prominent with the 14-3-3 proteins that have signaling and regulatory functions. Conversely, genes involved in protein translation and post-translational modification showed generally increased levels of transcription. These included translation elongation factor 2 and endoplasmic reticulum transport proteins. Overall, many changes in gene expression reflected changes beneficial to the virus while putting the cell at a metabolic disadvantage.