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United States Department of Agriculture

Agricultural Research Service

Title: Simple, Fast, and Cheap Cleanup Method for Determination of Aflatoxins in Major Agricultural Commodities by Liquid Chromatography

item Sobolev, Victor - AUBURN UNIVERSITY
item Dorner, Joe

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2001
Publication Date: March 1, 2002
Citation: --

Interpretive Summary: none required.

Technical Abstract: A simple, fast, reliable, and cheap chemical cleanup method for quantitation of aflatoxins in major important agricultural commodities using LC has been developed. Aflatoxins were extracted from a ground sample with methanol-water (80+20, v/v), and after a single cleanup step on a minicolumn packed with basic aluminum oxide, aflatoxins were quantified by LC equipped with a C18 column, a photochemical reactor, and a fluorescence detector. Water - methanol - 1-butanol (1400+720+25, v/v/v) served as the mobile phase. Recoveries of aflatoxins B1, B2, G1, and G2 from peanuts spiked at 5.0, 2.5, 7.5, 2.5 mg/kg were 87.2±2.3, 82.0±0.8, 80.0±1.8, and 80.4±2.8%, respectively. Similar recoveries, precision, and accuracy were achieved for corn, cottonseed, almonds, Brazil nuts, pistachios, and walnuts. The quantitation limit for aflatoxin B1 was 1 mg/kg. The minimal cost of the minicolumn allows for substantial savings compared with available commercial aflatoxin cleanup devices.

Last Modified: 4/19/2015
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