ARS | Publication request: CHARACTERIZATION OF THE PORCINE PEROXISOME PROLIFERATIVE ACTIVATED RECEPTOR GAMMA COACTIVATOR 1 (PPARGC1)

Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: April 1, 2002
Publication Date: August 10, 2002
Citation: Jacobs, K., Rohrer, G.A., Van Poucke, M., Piumi, F., Yerle, M., Mattheeuws, M., Van Zeveren, A., Peelman, L. 2002. Characterization of the porcine peroxisome proliferative activated receptor gamma coactivator 1 (PPARGC1) 28th Animal Genetics International Conference Proceedings. Abstract C025, p. 56-57.

Technical Abstract: Peroxisome proliferative activated receptor gamma coactivator 1 (PPARGC1) is a coactivator of nuclear receptors with an important function in adaptive thermogenesis. It influences genes involved in regulation of body weight and composition. Therefore, PPARGC1 can be considered as a candidate gene for carcass and meat quality traits. A BAC clone, isolated using a PPARGC1 PCR fragment has been mapped by FISH to Sscr8p21. A (CA)n-microsatellite (SGU0001) isolated from the BAC has been localised near marker SWR1101 on chromosome 8 by RH mapping and at the same position a marker KS195 (32.5 cM) by linkage mapping on the MARC reference family. Fragment analysis showed 8 different alleles in 73 unrelated animals of 5 different pig breeds (Meishan, Piétrain, Landrace, Large White and Czech Meat Pig). The coding exons of the porcine gene were sequenced and compared to human, mouse and rat sequences. The gene was scanned for polymorphisms. Allele frequencies were determined on unrelated animals of 5 different pig breeds. An AseI RFLP genotyping test was developed for a silent mutation detected in exon 8 and allele frequencies were determined on unrelated animals. The Meishan, Piétrain and Landrace breeds differ significantly from the Large White and the Czech Meat Pig breeds. Transcription of the gene was detected by RT-PCR in porcine duodenum, lung, ovary, brain, heart and adrenal gland tissues.