ARS | Publication request: THE MAIZE MAPPING PROJECT: COMPREHENSIVE GENETIC, PHYSICAL AND DATABASE RESOURCES FOR MAIZE

Submitted to: Maize Genetics Cooperation Newsletter
Publication Type: Other
Publication Acceptance Date: July 18, 2002
Publication Date: August 1, 2002
Citation: Coe Jr, E.H., Cone, K., Davis, G., Gardiner, J., Mcmullen, M.D., Polacco, M.L., Sanchez-Villeda, H., Wing, R., Soderlund, C., Chen, M. 2002. The maize mapping project: comprehensive genetic, physical and database resources for maize. Maize Genetics Cooperation Newsletter. V.76:78-79.

Technical Abstract: Progress in preparing an integrated genetic and physical map of maize is described. The strategy is to fingerprint bacterial artificial chromosome (BAC) fragments and assemble the fragments in a deep-coverage resource, accompanied by pegging with markers to confirm and to intercalate the assembled contigs. The markers are being anchored to the genetic map insofar as feasible, using restriction fragment polymorphism markers (RFLPs), simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs), and insertion-deletion polymorphisms (INDELs). Markers are being associated with BACs by hybridization on filters or by polymerase chain reactions with pooled BACs. Data are presented in the Maize Genome Database (MaizeDB), http://www.agron.missouri.edu/ and in the Maize Mapping Project (MMP) site, http://www.maizemap.org.