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United States Department of Agriculture

Agricultural Research Service

Title: The Maize Mapping Project: Comprehensive Genetic, Physical and Database Resources for Maize

Authors
item Coe Jr, Edward
item Cone, K - UNIV OF MISSOURI
item Davis, G - UNIV OF MISSOURI
item Gardiner, J - UNIV OF MISSOURI
item McMullen, Michael
item Schaeffer, Mary
item Sanchez-Villeda, H - UNIV OF MISSOURI
item Wing, R - CLEMSON UNIVERSITY
item Soderlund, C - CLEMSON UNIVERSITY
item Chen, M - CLEMSON UNIVERSITY

Submitted to: Maize Genetics Cooperation Newsletter
Publication Type: Other
Publication Acceptance Date: July 18, 2002
Publication Date: August 1, 2002
Citation: Coe Jr, E.H., Cone, K., Davis, G., Gardiner, J., Mcmullen, M.D., Polacco, M.L., Sanchez-Villeda, H., Wing, R., Soderlund, C., Chen, M. 2002. The maize mapping project: comprehensive genetic, physical and database resources for maize. Maize Genetics Cooperation Newsletter. V.76:78-79.

Technical Abstract: Progress in preparing an integrated genetic and physical map of maize is described. The strategy is to fingerprint bacterial artificial chromosome (BAC) fragments and assemble the fragments in a deep-coverage resource, accompanied by pegging with markers to confirm and to intercalate the assembled contigs. The markers are being anchored to the genetic map insofar as feasible, using restriction fragment polymorphism markers (RFLPs), simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs), and insertion-deletion polymorphisms (INDELs). Markers are being associated with BACs by hybridization on filters or by polymerase chain reactions with pooled BACs. Data are presented in the Maize Genome Database (MaizeDB), http://www.agron.missouri.edu/ and in the Maize Mapping Project (MMP) site, http://www.maizemap.org.

Last Modified: 4/16/2014
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