|Nishi, Sandra - USDA ARS VISITING SCI.|
Submitted to: World Congress of Genetics Applied in Livestock Production
Publication Type: Proceedings
Publication Acceptance Date: July 1, 2002
Publication Date: August 19, 2002
Citation: Dawson, H.D., Nishi, S., Beshah, E., Solano Aguilar, G., Zarlenga, D.S., Urban Jr, J.F., Lunney, J.K. 2002. Functional genomic assessment of porcine disease resistance: real time assays of porcine immune gene expression. World Congress of Genetics Applied in Livestock Production #13-41. Technical Abstract: Quantitation of genes involved in regulating disease resistance in swine is essential for identifying pigs, which are genetically resistant to infections. To this end we have developed quantitative TaqMan real-time PCR assays for assessing mRNA levels of immune regulatory molecules, specifically for porcine cytokines, including IFN-?, IL-4, IL-5, IL-10, IL-12 p35/p40, IL-15, IL18, and receptors, and the housekeeping genes, rRNA and HPRT. The assays were validated by 1) use of specific primer and probe sequences, 2) comparison of results with semiquantitative RT-PCR, and 3) expression in tissues from pigs infected with the parasites, Toxoplasma gondii or Ascaris suum. These assays will be used to compare immune phenotypes of lines of pigs to determine responses at different developmental stages and after vaccinations and infections.