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United States Department of Agriculture

Agricultural Research Service

Title: New Pollen-Specific Receptor Kinases Identified in Tomato, Maize and Arabidopsis: the Tomato Kinases Show Overlapping But Distinct Localizatoin Patterns on Pollen Tubes

Authors
item Kim, Hyun Uk - ARS-UCB PLNT GENE EXP CTR
item Cotter, Robyn - ARS-UCB PLNT GENE EXP CTR
item Johnson, Sheila - ARS-UCB PLNT GENE EXP CTR
item Senda, Mineo - ARS-UCB PLNT GENE EXP CTR
item Dodds, Peter - ARS-UCB PLNT GENE EXP CTR
item Kulikauskas, Rima - ARS-UCB PLNT GENE EXP CTR
item Tang, Weihua - ARS-UCB PLNT GENE EXP CTR
item Ezcurra, Ines - ARS-UCB PLNT GENE EXP CTR
item Herzmark, Paul - UCSF SAN FRANCISCO
item McCormick, Sheila

Submitted to: Plant Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 31, 2001
Publication Date: September 1, 2002
Citation: KIM, H., COTTER, R., JOHNSON, S., SENDA, M., DODDS, P., KULIKAUSKAS, R., TANG, W., EZCURRA, I., HERZMARK, P., MCCORMICK, S.M. NEW POLLEN-SPECIFIC RECEPTOR KINASES IDENTIFIED IN TOMATO, MAIZE AND ARABIDOPSIS: THE TOMATO KINASES SHOW OVERLAPPING BUT DISTINCT LOCALIZATOIN PATTERNS ON POLLEN TUBES. PLANT MOLECULAR BIOLOGY. 2002. 33:12:1641-1650.

Interpretive Summary: We characterized pollen-specific receptor kinases from tomato and from maize. We show that all three tomato kinases localize to the pollen tube wall. Our results reveal the existence of a distinct pollen-specific receptor kinase gene family whose members are likely to be involved pollen tube growth.

Technical Abstract: We previously characterized LePRKl and LePRK2, pollen-specific receptor kinases from tomato (Mushietti et al., 1998). Here we identify a similar receptor kinase from maize, ZmPRKl, that is also specifically expressed late in pollen development, and a third pollen receptor kinase from tomato, LePRK3. LePRK3 is less similar to LePRKl and LePRK2 than either is to each other. We used immunolocalization to show that all three LePRKs localize to the pollen tube wall, in partially overlapping but distinct patterns. We used RT-PCR and degenerate primers to clone homologs of the tomato kinases from other Solanaceae. We deduced features diagnostic for pollen receptor kinases and used these criteria to identify family members in the Arabidopsis database. RT-PCR confirmed pollen expression for five of these Arabidopsis candidates, two of these are clearly homologs of LePRK3. Our results reveal the existence of a distinct pollen-specific receptor kinase gene family whose members are likely to be involved in perceiving extracellular cues during pollen tube growth.

Last Modified: 10/1/2014
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