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United States Department of Agriculture

Agricultural Research Service

Title: Cloning and Expression of Envelope Gene of Subgroup J Avian Leukosis Virus

Authors
item Qin, Aijian - YANGZHOU UNIV CHINA
item Cui, Zhizhong - YANGZHOU UNIV CHINA
item Lee, Lucy
item Fadly, Aly

Submitted to: Virologica Sinica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 6, 2001
Publication Date: December 6, 2001
Citation: Qin, A., Cui, Z., Lee, L.F., Fadly, A.M. 2001. Cloning and expression of envelope gene of subgroup j avian leukosis virus. Virologica Sinica. 5:425-428.

Interpretive Summary: Subgroup J avian leukosis virus (ALV-J) is an emerging economically important virus infection that can cause cancer-like disease and other production problems in meat-type chickens. We generated large quantities of a specific reagent termed ALV-J envelope glycoprotein that can be used for the diagnosis of this important virus infection. The information obtained from this research is of great interest to scientists in industry and academia and will aid in developing diagnostic kits for detection of ALV-J infection in the poultry farm.

Technical Abstract: Avian leukosis virus subgroup J (ALV-J)was identified in the l990's, and causes mye1ocytic myeloid leukosis in meat-type chicken. The envelope (env)gene of ADOL-4817 strain of ALV-J was amplified by po1ymerase chain reaction (PCR)and cloned into TA vector. The size of env gene is about 1.7 kb. A transfer vector pBac4817-env was constructed by ligation between env gene and pBlue Bac4 plasmid DNA. Co-transfection of Bac-N-Blue baculovirus DNA and pBac4817-env DNA in Sf9 cells, a recombinant bacu1ovirus(rBac4817-env-2)was obtained. Immunofluorescense analysis results showed that the recombinant env gene products were expressed in Sf9 cells infected with rBac4817-env2. The molecular weight of expressed protein was about 90-94kDa in Western blot analysis. The recombinant gene products induced antibodies to ALV-J viruses in chickens. These results suggested that the expressed recombinant env gene products will be useful in studying the biological characterization of env gene.

Last Modified: 10/24/2014
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