Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Other
Publication Acceptance Date: September 5, 2002
Publication Date: N/A
Technical Abstract: Full genomics programs are unlikely to occur for minor crops in the foreseeable future, yet the wealth of genomic information available could be utilized if simple and cost effective strategies could capitalize on combining gene expression with trait inheritance. One approach is to use ESTs (Expressed Sequence Tags) to locate genes to linkage groups. Rather than design primers to amplify each EST separately and individually, an approach to streamline the process by developing amplicons that can then be queried using a single primer was tested. Results suggested that a restriction enzyme by EST primer combination can be useful for detecting segregation at actively expressed genes, and that in beet, a reasonable level of polymorphism can be expected. Across 18 restriction enzyme by EST combinations tested, only three failed to show at least one polymorphism. Of the one to 16 amplified fragments detected in each of the 15 polymorphic combinations, the proportion of polymorphic bands ranged from 10 to 100 percent. In order to demonstrate linkage with other molecular markers, segregation was compared in a sugar x red beet segregating population. Nine tested polymorphisms were linked with other types of markers on six of the nine beet linkage groups. Protocols are relatively straightforward and amenable to high-throughput operations. EST primer design and choice of restriction enzyme appear to be most critical for ensuring success.