ARS | Publication request: MOLECULAR PHYLOGENETIC RELATIONSHIPS AMONG DIABROTICA SPECIES (ACCESSION NO. AF195196)

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: May 11, 2001
Publication Date: May 11, 2001
Citation: Roehrdanz, R.L. 2001. Molecular phylogenetic relationships among Diabrotica species. GenBank. Accession no. AF195196.

Technical Abstract: Corn rootworms of the genus Diabrotica (Coleoptera: Chrysomelidae) are the most serious pest of corn in midwestern United States. Despite their economic importance, phylogenetic relationships within the genus remain unclear. Phylogenetic analysis of five Diabrotica was undertaken using DNA sequences of the nuclear rDNA first internal transcribed spacer region (ITS1) and a portion of the mtDNA cytochrome oxidase I and II genes (COI/COII). Parsimony and neighbor-joining cladograms indicated that southern corn rootworm is sister to banded cucumber beetle, whereas, northern corn rootworm forms a distinct clade with western and Mexican corn rootworm. ITS1 and COI/COII were found to be useful markers for determining phylogenetic relationships among diabroticites.