Technical Abstract: Screening of peanut germplasm for resistance to Tomato spotted wilt virus (TSWV) has been largely inefficient due to the lack of a screening technique based on mechanical transmission of the virus under controlled environmental conditions. We have studied the reaction of three peanut cultivars (Georgia Green, Georgia Runner, C-99R) and one breeding line (C11-2-39) using a highly efficient mechanical inoculation procedure. The disease response was studied at two temperature regimes, 25 to 30°C (low temperature) and 30 to 37°C (high temperature). Based on percent transmission, symptomatology, distribution of TSWV and relative levels of TSWV nucleocapsid (N) protein, Georgia Runner and Georgia Green were found to be susceptible whereas, C-99R and C11-2-39 were resistant. Of all the four genotypes tested, Cl1-2-39 had the highest level of resistance to TSWV. The results correlated with the field performance of the genotypes except in the case of Georgia Green which could not be distinguished from TSWV susceptible Georgia Runner. Exposure of the inoculated plants to higher temperature (30 to 37°C) resulted in a better resistant response as reflected by reduced systemic infection, localized symptom expression, restricted viral movement and reduced levels of TSWV antigen. This is the first report of differential response of peanut genotypes to TSWV using mechanical inoculation. The four peanut genotypes should be useful as reference 'standards' for the initial screening and identification of sources of TSWV resistance in peanut germplasm.