|Leeb, Tosso - IABG, HANNOVER, GERMANY|
Submitted to: Cytogenetics and Genome Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 2, 2002
Publication Date: June 30, 2002
Citation: Leeb, T., Rohrer, G.A. Characterization and chromosomal assignment of the porcine AHCY gene for S-adenosylhomocysteine hydrolase. 2002. Cytogenetics and Genome Research. 97:116-119. Interpretive Summary: The DNA sequence of S-adenosylhomocysteine hydrolase (AHCY) was determined for the pig. This gene was then positioned in the pig genome to chromosome 17 position 57 cM on the MARC genetic map. Placement of AHCY on the pig map enhances the pig-human comparative map. Furthermore, AHCY regulates important biological processes and may be responsible for observed differences in performance.
Technical Abstract: The gene for S-adenosylhomocysteine hydrolase (AHCY) is involved in the regulation of cellular methylation reactions. Here we report the cloning, sequencing, and chromosomal assignment of the porcine AHCY gene. The porcine AHCY gene consists of 10 exons spanning approximately 19 kb of genomic DNA. It encodes a protein of 432 amino acids that shows about 96% identity to the orthologous S-adenosylhomocysteine hydrolases from human, rat, and mouse. The porcine AHCY gene is located very close to the agouti signaling protein gene (ASIP) on SSC 17q21. The chromosomal localization was subsequently confirmed by the genetic mapping of an intragenic microsatellite that maps to 57 cM on the linkage map of SSC 17.