Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 4, 2002
Publication Date: April 4, 2002
Technical Abstract: Noncytopathic BVDV has the ability to establish non-apparent, persistent infections in both cell culture and in calves infected in the first trimester of pregnancy. The mechanism of development of tolerance of the infecting virus is unknown. To better understand the interactions of the host cell and virus, serial analysis of gene expression (SAGE) was used to develop a global picture of cellular events that take place following infection. Use of SAGE allows quantitation of virtually every transcript in a cell type without prior sequence information. Comparison of the SAGE data obtained from both noninfected and BVDV2-infected cells revealed a number of cellular changes that could be placed into distinct categories. Most obvious was the finding that the expression levels of both alpha and beta-tubulins were decreased, indicating potential cell division aberrations. Also showing lowered expression levels were genes encoding proteins involved in energy production and those critical for cap-dependent protein translation initiation. Expression of a number of genes involved in downstream protein translation and modification were upregulated. This included those involved in increasing translation efficiency and in nascent peptide transport into the endoplasmic reticulum. Many, but not all of the changes in gene expression that were observed were beneficial to the virus while placing the cell at a metabolic disadvantage.