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United States Department of Agriculture

Agricultural Research Service


item Okamura, Masashi
item Lillehoj, Hyun
item Raybourne, Richard - FDA
item Heckert, Robert - VA-MD REG. COL. VET. MED.
item Babu, Uma - FDA

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2002
Publication Date: N/A

Technical Abstract: The present study was conducted to investigate the effect of age on cell-mediated immune responses to different antigens from Salmonella serovar Enteritidis (SE) following vaccination with the commercially available heat-the killed bacterin. Eight-month- and 4-week-old chickens were given two subcutaneous injections with SE bacterin at 2 weeks apart. At 4, 7, 11, 14, 18, and 21 days post immunization (PI), spleen lymphocytes were stimulated with Concanavalin (Con) A, heat-killed SE (HK-SE), LPS, flagella, outer membrane protein (OMP), and porin and their proliferation measured using a non-radioactive proliferation assay (?CCK-8). Interferon ???IFN-g) and interleukin-2 (IL-2) produced by spleen cells stimulated with different SE antigens and secreted in the sera were assessed using a ELISA using antigen-specific monoclonal antibodies. Vaccinated chickens of 4-week of age (VY) showed higher proliferation response to LPS and flagella antigen, but not to the other antigens, compared to the unvaccinated young chickens, whereas 8-month-old chickens showed reduced proliferation response to SE antigens. Serum IFN-? and IL-2 levels were higher in the vaccinated birds compared to the age-matched control chickens regardless of the age group. The levels of IFN-? produced by the SE antigen-stimulated spleen cells were higher in the vaccinated young birds compared to the old chickens. IFN-? level in sera showed a significant increase after vaccination which corresponded to high IFN-? production by splenic cells stimulated with Con A and SE antigens. These results indicate that various antigenic components of SE bacteria induce IFN-? and IL-2 production, and younger chickens show better T-lymphocyte-mediated immunity following SE vaccination

Last Modified: 8/28/2016
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