ARS | Publication request: CLONING OF THREE DESICCATION REGULATED TRANSCRIPTS FROM DIAPAUSING COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA

Submitted to: Molecular Insect Science International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 27, 2002
Publication Date: N/A

Technical Abstract: Clones of three desiccation up-regulated transcripts (LdDes-1, -2 & -3) were isolated from diapausing Colorado potato beetle using suppression subtractive hybridization. Northern blot analysis demonstrated that LdDes-1, -2 & -3 are highly up-regulated in diapausing beetles and in desiccated nondiapausing adults. Transcript sizes are 1.35 Kb (LdDes-1), 0.8 Kb (LdDes-2) and 0.54 Kb (LdDes-3). A BlastX search revealed that LdDes-1 & -3 have identity to no known genes. The highest score for LdDes-2 was to the 28 kDa desiccation protein of Tenebrio molitor at 31% identity and 48% positives (identity + chemically equivalent). LdDes-1 encodes for a deduced protein of 34 kDa in size and is enriched (>9% each) for the amino acids glutamic acid, leucine, methionine, arginine and asparagine. The complete open reading frame for LdDes-3 has yet to be determined.