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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Sampling Methods for the Detection of Salmonellae on Whole Broiler Carcasses

Authors
item Simmons, M - UGA
item Fletcher, D - UGA
item Berrang, Mark
item Cason Jr, John

Submitted to: Poultry Science
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2002
Publication Date: July 1, 2002
Citation: Simmons, M., Fletcher, D.L., Berrang, M.E., Cason Jr, J.A. 2002. Comparison of sampling methods for the detection of salmonellae on whole broiler carcasses. Poultry Science. 81 (supp1):49 (abstract).

Technical Abstract: An experiment was conducted to determine the effect of sampling method on salmonellae recovery from the same carcass. One hundred fresh, whole broiler carcasses were purchased from retail outlets over a 5-week period (20 carcasses per week). After carcasses were aseptically removed from the packages and giblets removed, the carcasses were placed in sterile bags containing 400 ml of buffered peptone, shaken for 60 s, and then a 30 ml aliquot was removed (Sample A). An additional 130ml of buffered peptone was added to the bag with the same carcass, bringing the volume to 500 ml, after which the carcass was shaken and kept with the rinse (Sample B). Both samples were incubated for 24 h at 37 C. From each sample, 0.5 ml was placed into 10 ml each of Rappaport-Vassiliadis and tetrathionate (Hajna) broth and incubated at 42 C for 24 h. Each broth was then streaked onto BG Sulfa agar and modified lysine iron agar, and incubated for 24 h at 35 C. Suspect Salmonella colonies were inoculated on triple sugar iron and lysine iron agar slants and incubated at 35 C for 24 h. Presumptive positives were confirmed using Poly O and Poly H agglutination tests. Over the five week period, Sample A had 13 % salmonellae positive carcasses compared to 38 % for Sample B, from the same carcasses. Recovery ranged from 0/20 to 4/20 for Sample A, and 4/20 to 10/20 for Sample B over the 5 week period. These results indicate that when low numbers of salmonellae are expected, sampling methodology has a major influence on the identification of salmonellae-positive carcasses

Last Modified: 4/19/2014