|Castell, C - UNIVERSITY OF MINNESOTA|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 28, 2002
Publication Date: June 1, 2002
Citation: Castell, C., Samac, D.A., Szabo, L. 2002. Molecular variability and pathogenesis of Phoma medicaginis var. medicaginis isolates from Minnesota. Phytopathology. 92:S12. Technical Abstract: Phoma medicaginis var. medicaginis, the causal agent of spring black stem and leaf spot on alfalfa, causes serious losses in yield and quality worldwide. Alfalfa cultivars usually exhibit low to moderate levels of resistance. This study was undertaken to determine the molecular and pathogenic variability of P. m. var. medicaginis isolates from different environments and plant organs. A collection of 71 single spore isolates from northern and southern areas of Minnesota was used. Amplified Fragment Length Polymorphism (AFLP) analysis allowed separation of the majority of the isolates into two groups, which correlated with the northern and southern locations. Currently, additional isolates and primer-pair combinations are being evaluated in order to detect if there is organ specialization. In addition, isolates from different locations and organs are being tested to determine if there are differences in pathogenicity. This research will provide information on the population structure of Phoma medicaginis var. medicaginis on alfalfa.