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United States Department of Agriculture

Agricultural Research Service

Title: Microarray Analysis of Gene Expression in Soybean Roots

Authors
item Matthews, Benjamin
item Khan, Rana - UNIVERSITY OF MARYLAND
item Alkarouf, Nadim - GEORGE MASON UNIVERSITY
item Beard, Hunter
item Macdonald, Margaret
item Knap, Halina - CLEMSON UNIVERSITY

Submitted to: Proceedings of the IAPTC Conference
Publication Type: Abstract Only
Publication Acceptance Date: June 23, 2002
Publication Date: N/A

Technical Abstract: The soybean cyst nematode (SCN) is the major pest of soybean in the US and causes an estimated one billion dollars worth in damage each year. The defense response of soybean to SCN is a multigenic trait and varies depending upon the genotypes of soybean and SCN. The expression of over 3,000 soybean genes was monitored using microarrays to identify genes involved in the response of soybean to SCN. RNA was harvested from roots of soybean cv. Peking resistant to SCN race 3 and cv. Kent susceptible to SCN race 3, either not infected or at several time points after infection by SCN race 3 and was fluorescently labeled as cDNA for hybridization to the microarrays. A number of defense-related genes were at least 2-fold induced in the presence of SCN. Among the genes induced in soybean roots are defense-related genes, genes encoding potential regulatory factors such as phosphatases and transcription factors, and a number of genes encoding proteins of unknown function. These results provide some insights into the mechanisms used by soybean to respond to SCN attack. See our web site at http://bldg6.arsusda.gov/benlab/ for further information.

Last Modified: 8/22/2014
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