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United States Department of Agriculture

Agricultural Research Service

Title: A Comparison of Steam Distillation and Solid-Phase Microextraction (Spme)for Isolation of the Anemopsis Californica Volatiles

Authors
item Medina, A. - NEW MEXICO STATE UNIV
item Lucero, Mary
item ESTELL, RICHARD
item O'Connell, Mary - NEW MEXICO STATE UNIV

Submitted to: Society for Range Management, New Mexico Section Meeting
Publication Type: Abstract Only
Publication Acceptance Date: January 8, 2002
Publication Date: January 8, 2002
Citation: MEDINA, A.L., LUCERO, M.E., ESTELL, R.E., O'CONNELL, M.A. A COMPARISON OF STEAM DISTILLATION AND SOLID-PHASE MICROEXTRACTION (SPME)FOR ISOLATION OF THE ANEMOPSIS CALIFORNICA VOLATILES. SOCIETY FOR RANGE MANAGEMENT, NEW MEXICO SECTION WINTER MEETING. 2002. ABSTRACT P. 8.

Technical Abstract: Examination of the leaf volatiles of Anemopsis californica (Hook. & Arn.) was performed using steam distillation and solid-phase micro-extraction (SPME)in order to compare the results generated by the two techniques . Gas chromatography, followed by mass spectral or flame ionization detection, was used for identification or quantification, respectively. Data reveals complex volatile profiles regardless of sample preparation technique. However, key differences in composition obtained by SPME and steam distillation were seen for several compounds. Elemicin, methyleugenol, beta-pinene, thymol and 2-E-hexanal were more abundant in steam distilled than in SPME extracts. With the exception of 2-E-hexanal, all compounds detected by steam distillation were also detected by SPME. In addition, 10 volatiles seen in the SPME profile were not detected in steam distillates. Results indicate SPME is a useful tool for extraction of diverse volatiles from A. californica. However, targeted isolations of the compounds named above may be more efficient by steam distillation.

Last Modified: 7/28/2014