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United States Department of Agriculture

Agricultural Research Service

Title: Basal Nutrient Media Affects Axillary Shoot Proliferation & Preconditioningfor Adventitious Regeneration of Pears

item Bell, Richard
item Srinivasan, Chinnathambi
item Lomberk, Delores

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 12, 2002
Publication Date: June 1, 2002
Citation: Bell, R.L., Srinivasan, C., Lomberk, D.F. 2002. Basal nutrient media affects axillary shoot proliferation & preconditioningfor adventitious regeneration of pears. Meeting Abstract. 10th IAPTC&B Congress, Program & Abstracts, p.122-A, Abstract P-1354. 2002

Technical Abstract: The influence of the basal nutrient composition of plant tissue culture media on axillary shoot proliferation and their preconditioning effect on subsequent adventitious shoot regeneration from leaves was investigated. The goal was to improve both micropropagation and regeneration of 'Bartlett' and 'Beurre Bosc' pear cultivars. Driver-Kuniyuki Walnut (DKW) and Quorin and Lepoivre (QL) basal nutrient media were found to be superio to Murashige and Skoog (MS) and Woody Plant Medium (WPM) for axillary shoot proliferation. Shoots on WPM exhibited some chlorosis. Axillary shoot culture on DKW would be preferred to that on QL due to the production of excessively short, thin shoots on the latter medium. DKW also was superior to QL and MS for production of young expanding leaves for use as explants in adventitious regeneration. Leaf explants derived from shoot proliferation cultures grown on DKW or QL media produced more adventitious shoots than leaf explants from MS in both experiments.

Last Modified: 4/22/2015
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