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United States Department of Agriculture

Agricultural Research Service

Title: Detection of a Class I Integron in Multi-Drug Resistant Salmonella Niakhar

Authors
item Tankson, Jeanetta
item Cray, Paula
item Jackson, Charlene
item Gray, Jeffrey
item Barrett, John
item Headrick, Marcia - FDA-CVM

Submitted to: Emerging Infectious Diseases
Publication Type: Abstract Only
Publication Acceptance Date: January 25, 2002
Publication Date: March 24, 2002
Citation: TANKSON, J.D., CRAY, P.J., HUDSON, C.R., GRAY, J.T., BARRETT, J.B., HEADRICK, M. DETECTION OF A CLASS I INTEGRON IN MULTI-DRUG RESISTANT SALMONELLA NIAKHAR. EMERGING INFECTIOUS DISEASES. Session 50, abstract#15; p. 132

Technical Abstract: In the United States, Salmonella enterica serotype niakhar is a rarely isolated serotype of Salmonella. Between 1997 and 2000, the animal arm of the National Antimicrobial Resistance Monitoring System - Enteric Bacteria (NARMS) at USDA-ARS in Athens, GA assayed for antimicrobial resistance a total of 22,383 Salmonella isolates from various animals (swine, cattle, chickens, turkeys, cats, and dogs). Isolates originated from on-farm studies, veterinary diagnostic laboratories, or raw product collected from federally inspected slaughter and processing plants. Only 5 (0.02%) of these isolates were identified as niakhar (designated A-E). Of the five, B or D isolates were isolated in the Midwest (dairy cattle), A and E were isolated in the Southern United States (dairy cattle and dog). Antimicrobial resistance testing indicated that isolates A, B, and E were susceptible to all antibiotics tested; whereas isolate C was only resistant to ampicillin. Isolate D was resistant to ampicillin, chloramphenicol, ciprofloxacin, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim/sulfamethoxazole. Class I integrons (intI1) in Salmonella are known to harbor multiple resistance genes. Using PCR primers to the 5'conserved segment of intI1, a 568-bp fragment was amplified from isolate D. The integron from this isolate was localized to a ~ 12 kb XbaI fragment using PFGE. Further characterization of all isolates is ongoing. This is a first report of ciprofloxacin resistant Salmonella from NARMS as well as the first identification of intI1 in Salmonella niakhar.

Last Modified: 4/20/2014