|Donoghue, Dan - UNIV. OF ARKANSAS|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 22, 2002
Publication Date: June 4, 2002
Citation: SCHNEIDER, M.J., DONOGHUE, D.J. MULTIRESIDUE DETERMINATION OF FLUOROQUINOLONE ANTIBIOTICS IN EGGS USING LIQUID CHROMATOGRAPHY-FLUORESCENCE-MASS SPECTROMETRY. 4TH INTERNATIONAL SYMPOISIUM ON HORMONE AND VETERINARY DRUG RESIDUE ANALYSIS. ABSTRACT. 2002. P. 178. Technical Abstract: Fluoroquinolone antibiotics are currently used in both medical and veterinary applications. Use of these antibiotics in food animals has generated concern as the presence of these residues in food may contribute to increased microbial resistance in humans. Effective methods for analysis of fluoroquinolone residues in meat and related products, such as eggs, are thus needed. An efficient multiresidue method for determination of fluoroquinolone antibiotics in eggs has been developed, in which quantitation and confirmation are achieved simultaneously using fluorescence and mass spectrometry. With this method, eight fluoroquinolones were analyzed in fortified egg samples at levels of 10-200 ng/g. Recoveries for desethylene ciprofloxacin, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, orbifloxacin, sarafloxacin and difloxacin were generally in the range of 60-85%, with excellent RSDs. The elimits of quantitation ranged from 0.1-2 ng/g. Confirmation was achieved by monitoring the ratios of two prominent product ions in MS-MS (desethylene ciprofloxacin) or MS-MS-MS (all others). Utilization of this method in additional experiments with incurred egg samples will be described.