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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Fusarium Solani F. Sp. Glycines in Both Artificially Inoculated and Field Soybean Roots and Soil by DNA Direct Extraction and Pcr.

Authors
item Li, Shuxian - U OF ILL, URBANA
item Hartman, Glen

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2000
Publication Date: N/A

Technical Abstract: A PCR-based method was developed to detect Fusarium solani f. sp. glycines (FSG) the causal organism of soybean sudden death syndrome. Two pairs of primers were designed from the mitochondrial small subunit rRNA and the elongation factor 1-alpha genes. The PCR products were 439 and 237 bp respectively. Specificity of the primers was tested against 55 F. solani non-SDS causing isolates, 25 other soybean pathogens, and soybean. Sensitivity was determined to be 100 pg from pure template diluted with soybean DNA. The fungus was detected in artificially inoculated and field-grown soybean roots, and in soil by PCR using either single pairs of primers or the combination of two pairs of primers. Detection of FSG in soil samples collected from different soybean fields in Illinois were tested with a nested PCR approach that included two rounds of amplication. This PCR-based method has potential for identifying FSG and studying its distribution and occurrence.

Last Modified: 10/21/2014
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