Technical Abstract: Solanum chacoense Bitter is a wild species related to S. tuberosum L., the cultivated potato. The species is of interest to plant breeders because some individuals produce foliar-specific leptine glycoalkaloids which are believed to be factors in the resistance of S. chacoense to the Colorado potato beetle. Five related segregating F1 families of S. chacoense, developed from crosses between high x low and high x high leptine-producing selections, were utilized to construct molecular marker-based linkage maps using primarily AFLP, as well as a number of RAPD, SSR and DS-PCR markers. A framework of ordered markers was established for each family. Linkage groups from two sets of families generated from reciprocal crosses of high x low leptine producing parents were subsequently merged, resulting in two maps from high x low families and a single map from a high x high leptine cross. The results will be of interest for marker-based research in S. chacoense and related Solanaceous species and provide additional marker frameworks for integration into existing genetic maps.