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Title: DEVELOPMENT AND MAPPING OF MICROSATELLITE MARKERS DERIVED FROM CHICKEN CHROMOSOME-SPECIFIC LIBRARIES

Author
item AMBADY, S - UNIV OF MINNESOTA
item Cheng, Hans
item PONCE DE LEON, F A - UNIV OF MINNESOTA

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/24/2002
Publication Date: 12/31/2002
Citation: Ambady, S., Cheng, H.H., Ponce De Leon, F.A. 2002. Development and mapping of microsatellite markers derived from chicken chromosome-specific libraries. Poultry Science. 81:1644-1646.

Interpretive Summary: The field of genomics has great potential for all species, in addition to humans. Particularly relevant to animal agriculture, genomic research may enable scientists to identify genes that control complex traits such as meat yield, reproduction, and disease resistance. Prior to the identification of economically important genes, the appropriate research reagents such as genetic and physical maps need to be developed. In this paper, we describe a method to rapidly generate genetic markers from known chicken chromosomes. This result suggests it will be possible to develop efficiently genetic markers from defined locations, which in turn, enhances the ability to identify and refine the location of chromosomes containing the important genes. The poultry breeding industry will benefit from knowing where these genes are located, as the information would enable more rapid selection of superior animals for food and egg production. These advances would also benefit consumers in the form of more economical and safe poultry food products.

Technical Abstract: We have developed chromosome-specific painting probes and libraries for chicken macrochromosomes 1, 2, 3, and 4 by chromosome microisolation and microcloning. Fluorescent in situ hybridization results using the painting probes on normal chicken metaphase chromosomes indicated the purity and specificy of each probe. Chromosome-specific libraries for chicken macrochromosomes 1, 2, 3, and 4 were prepared in a phage vector. Fifty-two additional unique microsatellite markers of the (AC)n type were developed from these chromosome-specific libraries. These markers were mapped on the East Lansing reference population to increase the marker density on the four macrochromosomes. This study suggests that development of markers from chromosome-specific libraries is very useful for constructing high-density linkage maps for chicken macrochromosomes.