|Caldwell, D - TX A&M UNIVERSITY|
|Berghman, L - TX A&M UNIVERSITY|
|Mc Elroy, A - VIRGINIA TECH INSTITUTE|
|Hargis, B - UNIVERSITY OF ARKANSAS|
Submitted to: Comparative Biochemistry and Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 23, 2002
Publication Date: March 1, 2003
Citation: Moore, R.W., Caldwell, D.Y., Berghman, L.R., Caldwell, D.J., McElroy, A.P., Byrd II, J.A., Hargis, B.M. 2003. Effect of bursal anti-steroidogenic peptide and immunoglobulin G on neonatal chicken B-lymphocyte proliferation. Comparative Biochemistry and Physiology. 134:291-302. Interpretive Summary: In this study, attempts were made to produce antibodies, proteins which are produced by the immune system of animals for protection from infection. Antibodies can be made by injecting a purified protein from one species of animal into a different species of animal. In this study, a purified chicken protein called Bursal Anti-Steroidogenic Peptide (BASP) was injected into rabbits to produce antibodies for BASP. The protein is named BASP because it is found in an organ of chickens called the bursa of Fabricius which produces bursal cells that make antibodies. BASP may help to protect a chicken from infection. While attempting to find antibodies for BASP in injected rabbits, it was observed that rabbit blood containing antibodies also decreased production of a certain type of cell that may help fight infections. Results from this study suggest that the antibody produced may have an effect on the immune system in chickens. By understanding the immune system better, we may be able to help the animal' body to fight disease.
Technical Abstract: In attempts to identify antibodies for Bursal Anti-Steroidogenic Peptide (BASP), normal rabbit serum was observed to reduce phorbol ester- stimulated chicken B-lymphocyte proliferation. Experiments were designed to investigate the effects of IgG on B-lymphocyte proliferation. In experiment 1, 3% normal rabbit serum and 0.3 % hyperimmunized rabbit serum decreased B-lymphocyte proliferation. In experiment 2, 13 uM of rabbit IgG or the papain digestion products, Fab and Fc, decreased B-lymphocyte proliferation. Additionally, the combination of BASP and either Fab or Fc was observed to have a synergistic anti-proliferative effect. In experiment 3, 0.01 mg/mL of either commercially-purified rabbit or chicken IgG, or 1.0 mg/mL of rabbit or 0.01 mg/mL of chicken Fab, Fc, and the pepsin digestion product F(ab prime)2 was observed to have an anti- proliferative effect. No combined effects of BASP and IgG or IgG digest products were observed for this experiment. In experiment 4, 12 mg/mL purified egg yolk IgG or 1.2 mg/mL chicken Fab was found to suppress B- lymphocyte proliferation. Additionally, an additive effect of 12 mg/mL of IgG with BASP was again observed. The present studies suggest that IgG and its digestion products have anti-proliferative effects on B-lymphocyte proliferation in vitro and combined treatment with IgG and BASP may have an additive or synergistic effect on B-lymphocyte proliferation.