Submitted to: American Fishery Society (Fish Health Section) Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 8, 2002
Publication Date: February 11, 2002
Citation: Xu, D., Klesius, P.H., Shelby, R.A. 2002. Cutaneous Antibodies in Excised Skin from Channel Catfish Immune to Ichthyophthirius multifiliis. American Fishery Society (Fish Health Section) Proceedings. Charleston, SC. March 19-21, 2002. Page 57. Technical Abstract: This study determined whether cutaneous antibodies were present in the excised skin of channel catfish immune to Ichthyophthirius multifiliis(Ich). Theronts were immobilized on or near the excised skin from immune fish. The survival of immobilized theronts was significantly reduced after exposure for 8h to the culture of excised skin from immune fish. Culture fluids from excised skin of immune fish immobilized theronts with a peak in the immobilization titer at 24 PE. Immobility of theronts in the culture fluid from immune skin was removed after immunoabsorption with theronts. Indirect immunofluorescent staining of theronts treated with culture fluid from excised skin of immune fish revealed strong and uniform fluorescence on the cilia and cell surface of theronts. Western blot analysis of the culture fluid from immune fish revealed a 70 kDa band which corresponded to the molecular weight of catfish immunoglobulin heavy chain. The results of this study show that cutaneous antibodies to Ich theronts were present in and released from the excised skin from fish immune to Ich.