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United States Department of Agriculture

Agricultural Research Service

Title: From Fly to Flea Beetle: Identifying Non-Lepidopteran Semiochemicals with the Aid of Gc-Ead

item Cossé, Allard

Submitted to: International Society of Chemical Ecology Meeting
Publication Type: Proceedings
Publication Acceptance Date: July 12, 2001
Publication Date: N/A

Technical Abstract: Over the years, coupled gas chromatography-electroantennographic detection (GC-EAD) has seen numerous successful applications, including characterization of antennal responses to pheromones and host odor. Originally developed for lepidopteran antennal responses, this technique is used more and more for the detection of non-lepidopteran semiochemicals. The equipment and setup for lepidopteran GC-EAD is relatively standardized due to the more uniform sizes and shapes of lepidopteran antennae. However, the diversity in antennal morphology of non-lepidopteran antennae dictates some minor but often unreported adaptations to the method of GC-EAD analysis. Examples of such adaptations are discussed with newly identified pheromones of sawflies (Janus integer, Cephus cinctus), flea beetles (Aphthona spp., Phyllotreta spp.), a nitidulid (Colopterus truncates), and the cereal leaf beetle (Oulema melanopa).

Last Modified: 4/21/2015
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