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United States Department of Agriculture

Agricultural Research Service

Title: Standardized Methods for the Analysis of Isoflavones and Saponins in Processed Samples from Soybeans

item Berhow, Mark
item Cantrell, Charles
item Duval, Sandra
item Vaughn, Steven

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 30, 2001
Publication Date: N/A

Technical Abstract: The exact chemical composition of both the isoflavones and saponins found in processed products prepared from soybeans have been determined, but reproducible and accurate analytical methodology for the quantification of these compounds relies on the availability of pure standards and proper interpretation of the spectra. We have purified milligram to gram quantities of the three isoflavone glycosides and five "group B" saponin lycosides, as determined by LC-MS and NMR, from concentrated processing products produced from soy. These have been used to develop efficient UV-spectrophotometric HPLC methods to determine the percent weights of the isoflavones and the group B saponins in various processed soy products.

Last Modified: 4/19/2015