Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: April 20, 2002
Publication Date: March 20, 2002
Citation: Zeng, H. 2002. The molecular basis of selenite/seleno-methionine at physiological concentrations in promotion of HL-60 cell cycle progression [abstract]. The Federation of American Societies for Experimental Biology Journal. 16:A264. Technical Abstract: The elucidation of the mechanisms by which selenium regulates the cell cycle can lead to a better understanding of the nature of selenium- essentiality and disease-prevention. In this study, the effects of either selenite or seleno-methionine (0.25uM, physiological concentration) on HL- 60 cell cycle progression were examined in a serum-free media. Sodium selenite and seleno-methionine were equally effective and critical in cell segregation, morphology and promotion of cell cycle progression/growth. Cell-cycle analysis suggests that selenium-deficiency, but not selenium- adequacy, causes G2/M cell arrest. Relative cell cycle-related gene mRNA abundance determined by DNA array analysis suggested that c-Myc, cyclin C, PCNA, cdk1, cdk2, cdk4, cyclin B and cyclin D2 mRNA levels were higher in the cells supplemented with 0.25uM seleno-methionine than that of selenium- deficient cells. The increase of c-Myc mRNA level in selenium-supplemented cells was confirmed by RT-PCR assay. Furthermore, total cellular kinase protein (57%) was higher in selenium-supplemented cells than that of selenium-deficient cells. Collectively, these results suggest a novel role for selenite/seleno-methionine at physiological concentrations in up regulation of numerous cell cycle related gene expression and total cellular kinase protein, which are critical in cell cycle progression particularly in G2/M transition.