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United States Department of Agriculture

Agricultural Research Service

Title: Genomic Sample Sequencing of Three Bovine Shiga-Toxigenic E. Coli (Stec) O157, O111 and O26

item Bono, James
item Keele, John
item Kutish, Gerald
item Keen, James
item Laegreid, William

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 21, 2001
Publication Date: N/A

Technical Abstract: The recently completed genomic sequencing of E. coli O157:H7 EDL933 and Sakai isolates provides a wealth of information about the genes necessary for these shiga-toxin producing organisms to be human pathogens. Genomic sample sequencing of non-O157 shiga-toxigenic serotypes can be used to compare genomic content against both sequenced O157:H7 isolates and a non- shiga-toxin producing E. coli K12. We performed sample sequencing on bovine STEC of serotypes O157:H7, O111:NM, and O26:NM. A random total genomic library was generated from each serotype and sequenced to produce at least a 1X coverage of a 6 megabase genome. The O157:H7 isolate contains three sequencing reads with no homology to any sequence in the BLAST database and one sequencing read with a weak similarity to a transposase; four reads matched regions of the EDL933 genome but not the Sakai genome. The O111:NM isolate has 151 sequencing reads and the O26:NM isolate has 67 sequencing reads with no similarity to any nucleotide sequence in the BLAST database. Both the O26 and O111 isolates have many sequencing reads with homologous regions to either O157:H7 and/or K12. By comparing the genomic content of different shiga-toxin and non-shiga-toxin producing E. coli, we hope to better define the unique and essential elements of STEC.

Last Modified: 4/22/2015
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