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United States Department of Agriculture

Agricultural Research Service

Title: In Vitro Inhibition of Salmonella Enterica Serovars Choleraesuis and Typhimurium, Escherichia Coli F18, and Escherichia Coli O157:h7 by a Recombined Porcine Continuous-Flow Competitive Exclusion Culture

Authors
item HARVEY, ROGER
item DROLESKEY, ROBERT
item HUME, MICHAEL
item ANDERSON, ROBIN
item GENOVESE, KENNETH
item ANDREWS, KATHLEEN
item NISBET, DAVID

Submitted to: Current Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 31, 2001
Publication Date: July 1, 2002

Interpretive Summary: Competitive exclusion (CE) cultures are mixtures of beneficial intestinal bacteria that are given to animals in an attempt to prevent colonization by harmful bacteria. Salmonella and E. coli are bacteria found in swine that have the potential to cause disease in swine and humans. In this study, we evaluated the ability of a pig-origin CE culture to counteract the growth of Salmonella and E. coli in the laboratory. The CE culture successfully prevented the growth of the harmful bacteria. This is important because if it works in pigs, CE could reduce economic losses to the swine industry and improve the wholesomeness of pork in the food chain.

Technical Abstract: A competitive exclusion (CE) culture of porcine cecal bacteria was developed as a continuous-flow (CF) culture in chemostats, was designated RPCF, and was used as a model to determine its usefulness against colonization by Salmonella enterica serovars Typhimurium and Choleraesuis, and Escherichia coli strains F-18 and O157:H7 (933). At 24 h post-inoculation, reductions (P<0.05) of Choleraesuis, F-18, and O157:H7 were observed. Typhimurium was decreased (P<0.05) at 48 h post-inoculation, and by 120 h post-inoculation, all chemostats were negative for the four challenge organisms. These results demonstrate that RPCF cultures were able to inhibit the growth of Typhimurium, Choleraesuis, and E. coli strains F-18 and O157:H7 in vitro and suggest the potential for the use of CE in swine to prevent disease induced by these organisms.

Last Modified: 9/10/2014