ARS | Publication request: IDENTIFICATION OF FUNCTIONAL PROTEINS ENCODED BY THE MAIZE CHLOROTIC DWARF WAIKAVIRUS GENOME

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: March 26, 2001
Publication Date: N/A

Technical Abstract: The genome of the severe maize chlorotic dwarf waikavirus Ohio isolate (MCDV-S) is 11.8 kb long and encodes a single ca 400 kDa polyproteins that is posttranslationally cleaved into several smaller functional proteins. Regions of the genome encoding the three MCDV-S capsid proteins, a cysteine protease and RNA-dependent RNA polymerase are identified, but functions have not been assigned to proteins encoded by three separate regions of the virus genome. Three cDNAs corresponding to these regions were synthesized using RT-PCR and cloned into the pTrcHis plasmid. Sequence analysis of the cloned cDNA indicated more than 95% identity of MCDV-S with the previously characterized MCDV-type sequence. The His-tagged fusion proteins were produced in E. coli and affinity purified for raising antibodies in rabbits. The antibodies will be used for localization of the MCDV encoded proteins in leafhoppers and plants.