ARS | Publication request: USE OF REPETITIVE DNA SEQUENCES TO IDENTIFY GENETIC DIVERSITY IN HORDEUM AND AEGILOPS ACCESSIONS.

Submitted to: American Society of Agronomy
Publication Type: Abstract Only
Publication Acceptance Date: October 22, 2001
Publication Date: October 22, 2001
Citation: Burton, C.S., Hang, A., Bockelman, H.E. 2001. CD-ROM Use of repetitive dna sequences to identify genetic diversity in hordeum and aegilops accessions. American Society of Agronomy.

Interpretive Summary: Identification and characterization of plant species are important in germplasm collection and improvement. In this study, we used molecular biology techniques to characterize DNA from wild barley and wild wheat germplasm maintained in the USDA National Small Grains collection at Aberdeen, ID. Different primers were employed to amplify DNA from 17 wild barley and 17 wild wheat species. Two primers were able to differentiate all species investigated. These selected primers will be incorporated in future studies of accessions in the small grains collection.

Technical Abstract: Identification and characterization of plant species are important in germplasm collection and improvement. In this study, we used molecular biology techniques to characterize DNA from Hordeum and Aegilops germplasm maintained in the USDA National Small Grains Collection at Aberdeen, ID. Inter-simple sequence repeat (ISSR), simple sequence repeat (SSR), and general genomic amplification primers were used to analyze 17 Hordeum and 17 Aegilops species. The two general genomic amplification primers UBC892 and UBC900 differentiated all species investigated, based on genetic diversity evaluations and similarity dendograms. These selected primers will be incorporated in future studies of accessions in the small grains collection.