Submitted to: Antonie Van Leeuwenhoek
Publication Type: Review Article
Publication Acceptance Date: August 2, 2002
Publication Date: N/A
Technical Abstract: A competitive exclusion culture (CF3) containing a mixture of twenty- nine different bacterial isolates obtained from the cecae of broiler chickens was developed utilizing continuous-flow culture techniques. In experiments where day-old broiler chicks were provided CF3, and then challenged with 10,000 CFU Salmonella typhimurium greater than a 99% reduction in Salmonella cecal colonization levels was observed compared to experimentally infected controls that did not receive the culture. Similarly, CF3 has also been shown to protect experimentally infected broiler chicks from cecal colonization by S. enteritidis (Phage types 4 and 13), S. gallinarum, Listeria Monocytogenes, and Escherichia coli O157:H7. Additionally, groups of chicks provided CF3 that were experimentally infected with 100,000 CFU S. gallinarum had significantly less mortality compared to groups of chicks not provided CF3. A commercial product was developed from CF3 and is sold under the tradename PREEMPT. In a Food and Drug Administration approved, double blinded, pivotal field trial, chicks treated with PREEMPT had significantly fewer salmonellae than untreated chicks at end-of- growout. This product is the first of its kind available to the U.S. Poultry industry. Using similar technology a product has also been developed that decreases shedding of salmonellae in neonate and weaned pigs, and also has been shown to reduce mortality associated with enteropathogens in young pigs both in the laboratory and in a commercial swine herd.